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Structured Illumination Microscopy
Structured illumination microscopy (SIM) is a type of fluorescence microscope that incorporates a structured light generation device, such as a grating, spatial light modulator, or digital micromirror device (DMD), into the illumination path. By altering the spatial structure of the illumination light and using computational reconstruction, SIM overcomes the diffraction limit, achieving a lateral resolution close to or better than 100 nanometers and an axial resolution of about 300 nanometers, which is twice the resolution of traditional microscopes.
16 July 2024BestScope -
Köhler Illumination
In the process of microscopy imaging, the optical illumination system is essential. The commonly used illumination methods include critical illumination and Köhler illumination.
15 May 2024BestScope -
What is Microscope Immersion Oil?
The purpose of using immersion oil with a microscope is to change the refractive index of light when observing specimens with high-magnification objective lenses. This alteration allows more light to enter the objective lens, thereby enhancing resolution and enabling clearer observation and research of the specimen.
18 June 2024BestScope -
Microscope Beginners Guide: Definition, Parts and Functions.
The microscope was invented by the Dutch eyeglass merchant Zacharias Janssen in the late 16th century. This invention broke the limitations of human observation confined to the naked eye. Through one or more lenses, the microscope can magnify and observe tiny objects that are invisible to the naked eye. As a precision instrument, the microscope has been widely applied in fields such as education, medicine, biology, geology, materials science, forensic science, and electronic inspection.
22 February 2024BestScope -
What is a Compound Microscope?
Depending on differences in observation methods, applications, and operating principles, microscopes can be classified into various types. This article will start from the working principles of microscopes to help you better understand compound microscopes.
03 January 2024BestScope -
How are Microscopes Used in IVF, ICSI and IMSI?
Assisted reproductive technologies (ART) refer to a variety of medical procedures and technologies used to help individuals or couples become pregnant. ART involves the manipulation of human eggs, sperm, or embryos in a laboratory setting to help overcome infertility or genetic problems. Some common types of ART include in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI) and intracytoplasmic morphologically selected Sperm Injection (IMSI). These technologies offer the possibility for individuals or couples who have difficulty conceiving naturally.
04 December 2023BestScope
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